Effects of associated SCF and G-CSF on liver injury two weeks after liver damage: A model induced by thioacetamide administration

The present study aimed at investigating the beneficial effects of co-administering granulocyte colony–stimulating factor (G-CSF) and stem cell factor (SCF) in a model of chronic liver injury induced by thioacetamide (TAA). Biochemical and histopathologycal examinations were performed on serum and liver specimens. At the end of the treatment period, the rats were anesthetized with ether, serum was collected and sections of randomly selected fixed liver specimens from each group were embedded in paraffin and processed for light microscopy by staining individual sections with hematoxylineosin (HE) stain. Administration of a combination of G-CSF+SCF was carried out two weeks after the TAA treatment. Livers of rats treated with TAA alone exhibited damage, which was significantly less in the group treated with the combination of SCF and G-CSF. Albumin level was 2.35 (g/dl) in the G-CSF+SCF and 1.03 in the TAAalone group. These differences were statistically significant (P<0.05). Also, in the GCSF+SCF and TAA group the total protein means (7.16 versus 3.57 mg/dl, respectively) were higher than those of the TAA-alone group, and the differences were statistically significant (P<0.05). In the G-CSF+SCF and TAA group the total bilirubin content mean (0.15 versus 0.14 mg/dl, respectively) this difference was not statistically significant (P>0.05).